Accelerate to discover

Homepage>Discover>The importance of titrating Viability dyes on the Aurora Spectral Cytometer

Back to filter

Aug 16, 2024

We invite you to learn more about MERSCOPE Ultra Platform. Webinar featuring Rob Mathis (Global Instrument Product...

Single-Cell Genomics applications ? Ideal work for the WOLF gentle cell sorter

Aug 2, 2024

The field of genomics and computational biology has progressed immensely over the last decade and new single-cell...

Celloger Pro live-cell analyzer: miniaturized and powerful tool for Spheroids and Organoids research

Jul 31, 2024

Celloger live-cell analyzers, image and analyze your cells in real-time, inside your incubator. With its exceptional...

Webinar recording : BioFlux, the gold standard for Platelet Biology and Thrombosis research

Jul 29, 2024

Thrombosis, the underlying cause of major fatal conditions like ischemic stroke and myocardial infarction, poses a...

Oct 13, 2026

EV's and Nanoparticles in "Full Spectrum View" with the new Cytek ESP module

Jul 19, 2024

Cytek’s Enhanced Small Particle (ESP) Detection Option expands the capability of your Cytek Aurora or Cytek Northern...

Scaling Whole-Genome Sequencing to >50,000 single cells using cellenONE

Jul 16, 2024

cellenONE technology enabled the creation of tens of thousands of high-quality single-cell genomes, paving the way for...

Theranostics: From Mice to Men and Back

Jun 25, 2024

Recorded webinar
Presenters: Prof. Dr. Ken Herrmann and Prof. Dr. Katharina Lückerath – Moderator: Hannah Notebaert

Oct 13, 2026

Orion 2024 AACR poster: 17-plex single-step stain and imaging of cell Lung Carcinoma

Jun 21, 2024

RareCyte Orion is a benchtop, high resolution, whole slide multimodal imaging instrument. A combination of quantitative...

Show all topics (10)

The importance of titrating Viability dyes on the Aurora Spectral Cytometer

Jan 2, 2020

The first thing to note is how fixable viability dyes work. These viability dyes, like Zombie NIR, react with primary amine groups on proteins. That means that all cells will be stained with the viability dye because all cells express surface proteins. These dyes work as for viability because dead cells have permeable membranes, making the surface and intracellular proteins accessible to the viability dye and the fluorescence intensity much, much higher than live cells. Based on this information we can expect that if the manufacturer’s protocol is used (1 uL of Zombie NIR in 100 uL PBS), the Zombie NIR signature will be detected on all of the populations of cells.

Cytek Biosciences

Cytek provides compact and affordable flow cytometry instruments with multiplexing capability that streamlines workflows and delivers a high level of biological clarity for a majority of applications.

More info at:
https://cytekbio.com/